Biology Online Module Handbook

BL3322 Basic Biochemistry Laboratory

(BL3322 online module handbook version 21)

cover image
This module aims to provide students with the basic skills for independent laboratory work and an opportunity to develop academic skills such as: the design of experiments; logistic planning; the gathering and assessment of data; and the presentation of results. In addition, transferable skills such as group work, working to deadlines, numeracy, critical reading of peer-reviewed literature, and presenting material (oral and written) will be developed. Importantly, the course will also be an opportunity to meet and work with others in the honours cohort. The practical element will emphasise techniques in protein biochemistry.*

Credits: 10*

Semester: 1

Module Organiser
Dr Rona Ramsay
rrr@st-andrews.ac.uk
01334 463411
Pre-requisite Modules:
BEFORE TAKING THIS MODULE YOU SHOULD PASS BL2301 OR PASS BL2302 OR PASS BL2306. BEFORE TAKING THIS MODULE YOU MUST PASS BL2301 OR PASS BL2302 OR PASS BL2306*
Anti-requisite Modules:
IN TAKING THIS MODULE YOU CANNOT TAKE BL3000 OR TAKE BL3321. IN TAKING THIS MODULE YOU CANNOT TAKE BL3000 OR TAKE BL3321*
Post-requisite Modules:
*
Additional Module Information:
Please check MMS regularly for additional module information


Biology Teaching Office:
Room 1.17, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST

Email: bioteach@st-andrews.ac.uk      Tel: 01334  463602

BL3322: Timetable

Legend (not all modules have every event type):
lecture
tutorial
workshop
practical
other

Semester 1: Week -2

DATE & TIMEVENUESTAFFEVENT
Monday
04-09-2017
09:00 to 10:30
Purdie Building
LT-D
Dr Rona Ramsay
Dr Magnus Alphey
Ms Donna Pierz-Fennell
Lecture L1: Introduction and keeping a lab-book
2017-8_BL3322_L1
Safety
Monday
04-09-2017
10:30 to 16:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P1: Buffer, basic skills, spectrophotometric enzyme assay
2017-8_BL3322_P1
Monday
04-09-2017
16:00 to 17:00
Purdie Building
LT-D
Dr Catherine Adamson

Lecture L2: Reading scientific papers; literature introduction.
2017-8_BL3322_L2
Tuesday
05-09-2017
09:00 to 10:00
Biomolecular Sciences Building
BMS-LT
Dr Rona Ramsay

Lecture L3: Measuring proteins and enzymes
2017-8_BL3322_L3
Tuesday
05-09-2017
10:00 to 16:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P2: Measuring protein concentration and enzyme activity
2017-8_BL3322_P2
Tuesday
05-09-2017
16:00 to 17:00
Biomolecular Sciences Building
BMS-LT
Dr Rafael Guimaraes da Silva

Lecture L4: Kinetics, dynamics, and timescales in enzymology
2017-8_BL3322_L4
Wednesday
06-09-2017
09:00 to 10:00
Purdie Building
LT-D
sas28

Lecture L5: Protein identification by mass spectrometry
2017-8_BL3322_L5
Wednesday
06-09-2017
10:00 to 13:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P3: Protein separation
2017-8_BL3322_P3
Wednesday
06-09-2017
12:45 to 18:00
Medical and Biological Sciences Building
outside
Dr Catherine Adamson

Other O1: Group B: External visit, Kirkcaldy NHS
2017-8_BL3322_O1
Wednesday
06-09-2017
13:30 to 17:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P4: Group A: Measure activity and protein; pool fractions
2017-8_BL3322_P4
Thursday
07-09-2017
09:00 to 10:00
Purdie Building
LT-D
Dr Helder Ferreira

Lecture L6: Protein identification: Western blotting to GFP-labelling
2017-8_BL3322_L6
Thursday
07-09-2017
10:00 to 13:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P5: Group A: PAGE/start Western Blot
2017-8_BL3322_P5
Thursday
07-09-2017
10:00 to 13:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P6: Group B: Measure activity and protein; pool fractions
2017-8_BL3322_P6
Thursday
07-09-2017
12:45 to 18:00
Medical and Biological Sciences Building
outside
Dr Rafael Guimaraes da Silva

Other O2: Group A: External visit, Kirkcaldy NHS
2017-8_BL3322_O2
Thursday
07-09-2017
13:30 to 17:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P7: Group B: PAGE/start Western Blot
2017-8_BL3322_P7
Friday
08-09-2017
09:00 to 10:00
Purdie Building
LT-D
Dr Rona Ramsay
Ms Frances Entwistle
Miss Catherine Thomson
Lecture L7: Applying basic techniques: protein purification to drug design
2017-8_BL3322_L7
Friday
08-09-2017
10:00 to 16:00
Purdie Building
360
Dr Rona Ramsay
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Practical P8: Finish Western Blot; enzyme kinetics
2017-8_BL3322_P8
Friday
08-09-2017
16:00 to 18:00
Purdie Building
LT-D
Dr Catherine Adamson
Ms Frances Entwistle
Dr Rona Ramsay
Miss Catherine Thomson
Workshop W1: Student talks
2017-8_BL3322_W1
Friday
08-09-2017
18:00 to 19:00
Purdie Building
Coffee Room
Dr Rona Ramsay
Dr Catherine Adamson
Ms Frances Entwistle
Mrs Penny Hood
Miss Catherine Thomson
Other O3: Pizza
2017-8_BL3322_O3
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL3322: Reading List

Click for BL3322 reading list

BL3322: Assessment

1-hour Written Examination = 50%, Coursework = 50%*

(MMS assessment data cached: 17 November 2017 23:20:14.)


The following related information applies to all Biology modules:

School of Biology Marking Criteria: See School of Biology Undergraduate Handbook
Late submission of continuous assessment work:

All late submissions of coursework that do not require electronic submission should be made via the late submissions box in the Biomolecular Science Building (beside the Teaching Office)
Exam details: See School of Biology Undergraduate Handbook
Exam timetable: see http://www.st-andrews.ac.uk/students/academic/examinations/examtimetablescurrent/
Expected attendance: See School of Biology Undergraduate Handbook for detailed attendance requirements.
Good Academic Practice & Avoiding Academic Misconduct: See School of Biology Undergraduate Handbook
University Student Handbook: University Student Handbook
School and University regulations in the School and University Undergraduate Handbook relating to absence reporting, penalties and rules for late submission of work, extensions for coursework, return of coursework, S-coding, good academic practice and Academic Alerts.: School of Biology Undergraduate Handbook University Student Handbook
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

Who to ask

(Information in this section applies to all Biology Modules)

Questions about different aspects of the module should be directed to different people:

Questions about Contact
General teaching matters Biology Teaching Office ( bioteach@st-andrews.ac.uk )
Rescheduled or cancelled events Check your University email
Lecture or practical content The lecturer who presented the material
Completing assessed practical assignments The lecturer who set the assignment
Completing assessments Module Organiser (Dr Rona Ramsay rrr@st-andrews.ac.uk)
Marking on continuous assessment The Demonstrator or Module Organiser (Dr Rona Ramsay rrr@st-andrews.ac.uk)
Marking on exams Module Organiser (Dr Rona Ramsay rrr@st-andrews.ac.uk)
Rearranging practical days Grant Brown
Absence and/or extensions Module Organiser (Dr Rona Ramsay rrr@st-andrews.ac.uk) and the Biology Teaching Office ( bioteach@st-andrews.ac.uk )

Difficulties with academic progress which impact more than one module:

Year Coordinator

See School of Biology Undergraduate student handbook for list: http://biology.st-andrews.ac.uk/documents/UndergraduateHandbook.pdf

Overall performance, progress or future directions:

Advisor of Studies

Disability:

Disability Coordinator (Dr Jacqueline Nairn jn37@st-andrews.ac.uk)

Biology Teaching Office:

Room 1.17, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST

Email: bioteach@st-andrews.ac.uk

Tel: 01334 463602

BL3322: Contributing Staff

Dr Rona Ramsay

Dr Rona Ramsay
(Module Organiser)

Readerrrr@st-andrews.ac.uk
Dr Catherine AdamsonDr Catherine AdamsonLecturer in Molecular Medicinecsa21@st-andrews.ac.uk
Dr Magnus AlpheyX-Ray Facility Managermsa31@st-andrews.ac.uk
Ms Frances EntwistleMs Frances EntwistlePostgraduate Studentfe9@st-andrews.ac.uk
Dr Helder FerreiraDr Helder FerreiraLecturerhcf2@st-andrews.ac.uk
Dr Rafael Guimaraes da SilvaDr Rafael Guimaraes da SilvaResearch Fellowrgds@st-andrews.ac.uk
Mrs Penny HoodTeaching Technicianpah21@st-andrews.ac.uk
Ms Donna Pierz-FennellMs Donna Pierz-FennellSchool Managerdp61@st-andrews.ac.uk
Dr Rona RamsayDr Rona Ramsay
(Module Organiser)
Readerrrr@st-andrews.ac.uk
Miss Catherine ThomsonMiss Catherine ThomsonPostgraduate Studentcmt42@st-andrews.ac.uk
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL3322: Learning Outcomes

Students completing module BL3322 successfully should be able to:

  • Apply good working practices in the laboratory, including an awareness of issues relating to safety
  • Be confident in performing experiments
  • Acquire, organise and analyse data appropriately
  • Carry out standard biochemical techniques including spectrophotometric assays, chromatography, SDS-PAGE, Western blotting.
  • Record experiments in a lab-book
  • Read scientific literature critically
  • Have basic knowledge of how the NHS clinical laboratory works
  • Apply good working practices in the laboratory, including an awareness of issues relating to safety
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL3322: Acquired Skills

Practical Skills

  • Biomolecule isolation/characterisation
  • Buffers
  • Chromatography
  • Enzyme assay
  • Kinetic data analysis
  • Pipetting
  • Protein characterisation
  • Protein purification
  • Protein quantitation
  • SDS PAGE
  • Western Blot

Transferable Skills

  • Short group presentation on given topic (up to 15 min)
  • Handout (for presentation or poster)
  • Summary
  • Critically evaluating sources/information
  • Finding literature
  • Lab safety awareness
  • Problem-solving questions
  • Calculations/equations
  • Concentrations
  • Data presentation
  • Descriptive statistics
  • Dilutions
  • Draw a line of best fit
  • Linear regression
  • Non-linear fit
  • Produce graphs/figures
  • Produce tables
  • SI units
  • Use Excel
  • Volumes
  • Lab or field notebook
  • Critiquing experimental design
  • Designing experiments
  • Working in pairs/small groups
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

Policies

(Information in this section applies to all Biology Modules)