Dr Rafael Guimaraes da Silva:
Research Fellow

Dr Rafael Guimaraes da Silva

Dr Rafael Guimaraes da Silva
Biomolecular Sciences Building
University of St Andrews
North Haugh
St Andrews
KY16 9ST

tel: 01334 463496
room: B103

Related Content:

da Silva Lab website

School of Biology
Biology Postgraduate Recruitment Committee
Biomedical Sciences Research Complex

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Enzymes catalyse virtually all chemical reactions in living organisms, making their rates compatible with life. These proteins, and sometimes RNA’s, have evolved to utilize a range of strategies to achieve incredible rate enhancements in comparison with the corresponding non-catalysed reactions. The study of enzymatic mechanisms is fundamental to elucidate how enzymes work in physical and chemical terms, to guide the rational design of inhibitors, and to help engineer new activities. Here we apply techniques of biochemistry and physical chemistry to unravel the mechanisms of enzymatic reactions involved in bacterial histidine and 5-aminolevulinic acid biosynthesis and in human protein histidine phosphorylation. Particular emphasis is given to transition-state structure and to the role of protein dynamics in energy barrier crossing.   


  • Research Fellow (2015-Present) - University of St Andrews, St Andrews, UK

  • Principal Scientist (2014) - Pfizer, Groton, USA

  • Senior Scientist (2012-2013) - Pfizer, Groton, USA

  • Post-Doctoral Associate (2008-2012) - Albert Einstein College of Medicine, New York, USA

Adviser: Dr. Vern L. Schramm

  • D.Sc. - Biochemistry (2008) - Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

Adviser: Dr. Luiz A. Basso

  • M.Sc. - Biochemistry (2005) - Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

Adviser: Dr. Luiz A. Basso

  • B.Sc. - Biology (2002) - Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
source: symbiosis

Recent Publications:

Recent publications listed in research@st-andrews
McAllister, L, Butler, C, Mente, S, O'Neil, SV, Fonseca, K, Piro, J, Cianfrogna, J, Foley, T, Gilbert, A, Harris, A, Helal, CJ, Johnson, DS, Montgomery, JI, Nason, DM, Noell, S, Pandit, J, Rogers, BN, Samad, TA, Shaffer, CL, da Silva, RG, Uccello, DP, Webb, D & Brodney, MA 2018, 'Discovery of trifluoromethyl glycol carbamates as potent and selective covalent monoacylglycerol lipase (MAGL) inhibitors for treatment of neuroinflammation' Journal of Medicinal Chemistry, vol 61, no. 7, pp. 3008–3026. DOI: 10.1021/acs.jmedchem.8b00070
Ramos, LM, Guido, BC, Nobrega, CC, Correa, JR, Silva, RG, de Oliveira, HCB, Gomes, AF, Gozzo, FC & Neto, BAD 2013, 'The Biginelli reaction with an imidazolium-tagged recyclable iron catalyst: kinetics, mechanism, and antitumoral activity' Chemistry - A European Journal, vol 19, pp. 4156-4168. DOI: DOI: 10.1002/chem.201204314