Profile

Dr Jens Tilsner:
Lecturer

Dr Jens Tilsner

Dr Jens Tilsner
Biomolecular Sciences Building
University of St Andrews
North Haugh
St Andrews
KY16 9ST
Fife
UK

tel: 01334 464829
fax: 2595
room: 3.17
email: jt58@st-andrews.ac.uk

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School of Biology
Biomedical Sciences Research Complex

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My lab investigates the interactions of plant RNA viruses with host cell membranes during replication and cell-to-cell transport.

Plant viruses are a major threat to food security. Unlike viruses infecting animals, they need to cross the barrier of the cell wall during infection. This step constitutes a severe bottleneck in the infection process, and is therefore a promising target for the development of new antiviral crop protection strategies.

RNA viruses assemble replication complexes on membrane surfaces and extensively reorganise membrane composition and shape in the process. Membrane interactions also play a role in plant viruses intercellular transport because this occurs through cell wall-spanning nanopores called plasmodesmata that consist of two concentric membrane tubules. Recently, I discovered that in some plant viruses, the processes of replication and movement are coupled in modified membrane structures at the entrances of the plasmodesmata.

We use a combination of confocal and super-resolution microscopy of RNA and proteins, protein biochemistry, in vitro reconstitution of membrane-bound protein complexes, and lipidomics to understand how plant virus-encoded proteins modify cellular membranes for replication and movement, and how this knowledge can be translated into crop protection strategies and biotechnology applications.

 



source: symbiosis


Recent Publications:

Recent publications


5  (of 32 published available) for jt58. (source: University of St Andrews PURE)
Please click title of any item for full details.

2018 (1/2)
Frontiers in Plant Science
vol.9 
(Article)
Efficient detection of long dsRNA in vitro and in vivo using the dsRNA binding domain from FHV B2 protein
Baptiste Monsion, Marco Incarbone, Kamal Hleibieh, Vianney Poignavent, Ahmed Ghannam, Patrice Dunoyer, Laurent Daeffler, Jens Tilsner, Christophe Ritzenthaler 
Keywords: dsRNA, Virus, Viral factories, Replication complexes, Detection, Plant, Insect, Nicotiana benthamianaQH301 Biology, QR355 VirologyNDAS
2018 (6)
Current Opinion in Plant Biology
vol.43 pp.82-88
(Review article)
Hitchhikers, highway tolls and roadworks
Svetlana Y. Folimonova, Jens Tilsner 
Keywords: QR355 Virology, SB Plant culture
2017 (12/6)
Nature Plants
vol.3 
(Article)
Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves
William J. Nicolas, Magali S. Grison, Sylvain Trépout, Amélia Gaston, Mathieu Fouché, Fabrice P. Cordelières, Karl Oparka, Jens Tilsner, Lysiane Brocard, Emmanuelle M. Bayer 
Keywords: QH301 BiologyPlant ScienceNDAS
2016 (8)
Traffic
vol.17 pp.923-939
(Article)
"2A-like" signal sequences mediating translational recoding
Garry Alec Luke, Pablo de Felipe, Lin Ruan, Jonathan Cope, John Nicholson, Andriy Sukhodub, Jens Tilsner, Martin Denis Ryan 
Keywords: Translational recoding, 2A, Signal sequence, Secretory pathway, Dual protein targetingQH301 Biology
2016 (4)
Annual Review of Plant Biology
vol.67 pp.337-364
(Review article)
Staying tight
Jens Tilsner, William Nicolas, Abel Rosado, Emmanuelle M. Bayer 
Keywords: Plasmodesmata, Intracellular trafficking, Non-cell-autonomous, Callose, Membrane contact site, Calcium signalingQH301 Biology