Location: room B122
The Leica TCS SP8 is fully equipped with Leica ‘s modern imaging enhancements.
The system incorporates a motorised inverted DMI 6000 microscope, which has 4 objectives.
|Magnification||Leica Type||NA/mm||Immersion medium|
|X10||HC PL FLUOTAR||0.3||AIR|
|X20||HC PL APO||0.7||WATER/GLYCEROL/OIL|
|X40||HC Plan APO||1.3||OIL|
|X63||HC Pl APO||1.4||OIL|
There is also a motorised x/y-stage allowing for multi-position imaging and tiling.
For eyepiece visualization there are three fluorescent filters
- Blue (A = x340-380, dichroic 400, LP425)
- Green (I3 = xBP470-490, dichroic 510, mLP515)
- Red (N2.1 = x515-560, dichroic 580, mLP590)
The SP8 has a Tandem scanner system, which provides for Conventional scanning, or Resonant scanning (8 000 Hz) – 28 fps at 512*512 for fast live cell imaging.
It contains four lasers, allowing for excitation in the following wavelengths: 405 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, 543nm, 561nm, 594nm and 633 nm.
Up to four fluorophores plus a bright field setting can be imaged in parallel or sequentially. There are four interactive emission detectors, enabling the user flexibility in choosing the wavelengths to detect; two of which are highly sensitive (GaAsP) allowing the imaging of faint signals.
To facilitate live cell imaging there is an environmental chamber with temperature control and a macro mode function within the LASAF software for complex time-lapse experiments.