Location: room B106
The Leica TCS SL confocal microscope can be considered a standard confocal imaging system, which incorporates an easy to use, configurable interface with drop down menus for software utilities (user profiles and Leica templates)
The system includes a DM IRE 2 Leica inverted microscope equipped with the following objectives:
|Magnification||Leica Type||NA/mm||Immersion medium|
|X10||HC PL Fluotar||0.3||Air|
|X20||HC Pl Fluotar||0.5||Air|
|X40||HCX PL APO||1.25||Oil|
|X63||HCX PL APO||1.4||Oil|
The microscope also contains a manual filter block that provides for the initial visualisation of the specimen.
The TCS SL Confocal system carries 3 lasers:
|Multiline Argon laser458 nm: CFP488 nm: FITC, Alexa 488, CY2, GFP, Fluojade-4, Calcein514 nm: YFP|
|543 nmGreen Helium-Neon laser (Gre-Ne)Cy3, RFP, Alexa 546, Alexa 561, M Cherry|
|633 nmHelium-Neon laser (He-Ne)Cy5, DRAQ5, Mitrotracker Deep Red|
There is a three-channel, alignment free AOTF confocal scan head with a highly sensitive user adjustable sliding scale spectral excitation and detection system in the range of 400-800 nm. The software system includes configurable sequential imaging to eliminate cross talk in multiple labelling, This can be used in z stack acquisition or single image acquisition. Other modules included in the software are; Time-lapse imaging, volume rendering and animations, FRAP wizard and co-localisation analysis.