Publications

35 (of 35 /dk/atira/pure/researchoutput/status/published available) for rgds. (source: University of St Andrews PURE)
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Transition states for psychrophilic and mesophilic (R)-3-hydroxybutyrate dehydrogenase-catalyzed hydride transfer at sub-zero temperatures Teresa F. G. Machado, Miha Purg, Johan Åqvist, Rafael Guimaraes da Silva
Biochemistry 2021 vol. 60 pp. 2186–2194
Dissecting the mechanism of (R)-3-hydroxybutyrate dehydrogenase by kinetic isotope effects, protein crystallography, and computational chemistry Miha Purg, Verena Oehler, Johan Aqvist, Tracey Gloster, Rafael Guimaraes da Silva
ACS Catalysis 2020 vol. 10 pp. 15019–15032
An exceptionally potent inhibitor of human CD73 Christine E Bowman, Rafael Guimaraes da Silva, Amber Pham, Stephen W Young
Biochemistry 2019 vol. 58 pp. 3331-3334
Mapping the structural path for allosteric inhibition of a short-form ATP phosphoribosyltransferase by histidine Magnus Stephen Alphey, Rafael Guimaraes da Silva
Biochemistry 2019 vol. 58 pp. 3078-3086
Allosteric activation shifts the rate-limiting step in a short-form ATP phosphoribosyltransferase Rozanne Stroek, Clarissa Melo Czekster, Jennifer S. Hirschi, Rafael Guimaraes da Silva
Biochemistry 2018 vol. 57 pp. 4357-4367
Catalytic and anticatalytic snapshots of a short-form ATP phosphoribosyltransferase Magnus Stephen Alphey, Eoin Rory Gould, Gordon John Florence, Jim Naismith, Rafael Guimaraes da Silva
ACS Catalysis 2018 vol. 8 pp. 5601-5610
Discovery of trifluoromethyl glycol carbamates as potent and selective covalent monoacylglycerol lipase (MAGL) inhibitors for treatment of neuroinflammation Laura McAllister, Christopher Butler, Scot Mente, Steven Neil, Kari Fonseca, Justin Piro, Julie Cianfrogna, Timothy Foley, Adam Gilbert, Anthony Harris, Christopher J. Helal, Douglas S. Johnson, Justin I. Montgomery, Deane M. Nason, Stephen Noell, Jayvardhan Pandit, Bruce N. Rogers, Tarek A. Samad, Christopher L. Shaffer, Rafael Guimaraes da Silva, Daniel P. Uccello, Damien Webb, Michael A. Brodney
Journal of Medicinal Chemistry 2018 vol. 61 pp. 3008–3026
Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction Tracey Gloster, Rafael Guimaraes da Silva
Biochemistry 2018 vol. 57 pp. 6757-6761
Kinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferase Rozanne Stroek, P. D. Talbot, M. K. Glok, K. E. Bernas, Eoin Rory Gould, Magnus Stephen Alphey, Gordon John Florence, Jim Naismith, Rafael Guimaraes da Silva
Biochemistry 2017 vol. 56 pp. 793-803
A continuous and direct assay to monitor leucine-rich repeat kinase 2 activity Rafael Guimaraes da Silva, Kieran F Geoghegan, Xiayang Qiu, Ann Aulabaugh
Analytical Biochemistry 2014 vol. 450 pp. 63-69
Selective mitochondrial staining with small fluorescent probes: importance, design, synthesis, challenges and trends for new markers Brenno AD Neto, Jose R Correa, Rafael Guimaraes da Silva
RSC Advances 2013 vol. 3 pp. 5291-5301
The Biginelli reaction with an imidazolium-tagged recyclable iron catalyst: kinetics, mechanism, and antitumoral activity Luciana M Ramos, Bruna C Guido, Catharine C Nobrega, Jose R Correa, Rafael Guimaraes da Silva, Heibbe CB de Oliveira, Alexandre F Gomes, Fabio C Gozzo, Brenno AD Neto
Chemistry - A European Journal 2013 vol. 19 pp. 4156-4168
Constrained bonding environment in the Michaelis complex of Trypanosoma cruzi uridine phosphorylase Rafael Guimaraes da Silva, David R Kipp, Vern L Schramm
Biochemistry 2012 vol. 51 pp. 6715-6717
Synthesis, properties and highly selective mitochondria staining with novel, stable and superior benzothiadiazole fluorescent probes Brenno AD Neto, Pedro HPR Carvalho, Diego CBD Santos, Claudia C Gatto, Luciano M Ramos, Nathalia M de Vasconcelos, Jose R Correa, Maisa B Costa, Heibbe CB de Oliveira, Rafael Guimaraes da Silva
RSC Advances 2012 vol. 2 pp. 1524-1532
Arsenate and phosphate as nucleophiles at the transition states of human purine nucleoside phosphorylase Rafael Guimaraes da Silva, Jennifer S Hirschi, Mahmoud Ghanem, Andrew S Murkin, Vern L Schramm
Biochemistry 2011 vol. 50 pp. 2701-2709
Femtosecond dynamics coupled to chemical barrier crossing in a Born-Oppenheimer enzyme Rafael Guimaraes da Silva, Andrew S Murkin, Vern L Schramm
Proceedings of the National Academy of Sciences of the United States of America 2011 vol. 108 pp. 18661-18665
Mass-dependent bond vibrational dynamics influence catalysis by HIV-1 protease David R Kipp, Rafael Guimaraes da Silva, Vern L Schramm
Journal of the American Chemical Society 2011 vol. 133 pp. 19358-19361
Transition-state analysis of Trypanosoma cruzi uridine phosphorylase-catalyzed arsenolysis of uridine Rafael Guimaraes da Silva, Mathew J Vetticatt, Emilio F Merino, Maria B Cassera, Vern L Schramm
Journal of the American Chemical Society 2011 vol. 133 pp. 9923-9931
Uridine phosphorylase from Trypanosoma cruzi: kinetic and chemical mechanisms Rafael Guimaraes da Silva, Vern L Schramm
Biochemistry 2011 vol. 50 pp. 9158-9166
Purine nucleoside phosphorylase activity and expression are upregulated in sites affected by periodontal disease Eraldo L Batista Jr, Candida Deves, L Ayub, Rafael Guimaraes da Silva, LCC Filho, Luiz A Basso, Diogenes S Santos
Journal of Periodontal Research 2010 vol. 45 pp. 664–671
Mycobacterium tuberculosis beta-ketoacyl-ACP reductase: alpha-Secondary kinetic isotope effects and kinetic and equilibrium mechanisms of substrate binding Rafael Guimaraes da Silva, Leonardo Rosado, Diogenes S Santos, Luiz A Basso
Archives of Biochemistry and Biophysics 2008 vol. 471 pp. 1-10
Kinetic and chemical mechanisms of shikimate dehydrogenase from Mycobacterium tuberculosis Isabel O Fonseca, Rafael Guimaraes da Silva, Claudia Fernandes, Osmar N de Souza, Luiz A Basso, Diogenes S Santos
Archives of Biochemistry and Biophysics 2007 vol. 457 pp. 123-133
Purine Nucleoside Phosphorylase: A Potential Target for the Development of Drugs to Treat T-Cell- and Apicomplexan Parasite-Mediated Diseases Rafael Guimaraes da Silva, Jose E S Nunes, Fernanda Canduri, J C Borges, L M Gava, F B Moreno, Luiz A Basso, Diogenes S Santos
Current Drug Targets 2007 vol. 8 pp. 413-422
Beta-Ketoacyl-Acyl Carrier Protein (ACP) Reductase: Kinetic and Chemical Mechanisms Rafael Guimaraes da Silva, Luiz P S de Carvalho, John S Blanchard, Diogenes S Santos, Luiz A Basso
Biochemistry 2006 vol. 45 pp. 13064-13073
Functional shikimate dehydrogenase from Mycobacterium tuberculosis H37Rv: Purification and characterization Isabel O Fonseca, Maria L B Magalhaes, Jaim S Oliveira, Rafael Guimaraes da Silva, Maria A Mendes, Mario S Palma, Diogenes S Santos, Luiz A Basso
Protein Expression and Purification 2006 vol. 46 pp. 429-437
Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine Rafael Guimaraes da Silva, Jose H Pereira, F Canduri, WF de Azevedo, LA Basso, DS Santos
Archives of Biochemistry and Biophysics 2005 vol. 442 pp. 49-58
Structure of human PNP complexed with ligands Fernanda Canduri, Rafael Guimaraes da Silva, D M dos Santos, MS Palma, LA Basso, DS Santos, WF de Azevedo
Acta Crystallographica. Section D, Biological crystallography 2005 vol. 61 pp. 856-862
Purine Nucleoside Phosphorylase Activity in Rat Cerebrospinal Fluid Rafael Guimaraes da Silva, DS Santos, LA Basso, JP Oses, S Wofchuk, LVC Portela, DO Souza
Neurochemical Research 2004 vol. 29 pp. 1831-1835
Structures of human purine nucleoside phosphorylase complexed with inosine and ddI F Canduri, DM dos Santos, Rafael Guimaraes da Silva, MA Mendes, LA Basso, Palma MS, WF de Azevedo, DS Santos
Biochemical and Biophysical Research Communications 2004 vol. 313 pp. 907-914
Cloning, overexpression, and purification of functional human purine nucleoside phosphorylase Rafael Guimaraes da Silva, LPS de Carvalho, JS Oliveira, CA Pinto, MA Mendes, MS Palma, LA Basso, DS Santos
Protein Expression and Purification 2003 vol. 27 pp. 158-164
Crystal structure of human PNP complexed with guanine WF de Azevedo, F Canduri, D dos Santos, J Pereira, MVB Dias, Rafael Guimaraes da Silva, MA Mendes, LA Basso, MS Palma, DS Santos
Biochemical and Biophysical Research Communications 2003 vol. 312 pp. 767-772
Crystal structure of human purine nucleoside phosphorylase at 2.3 A resolution WF de Azevedo, F Canduri, DM dos Santos, Rafael Guimaraes da Silva, JS Oliveira, LPS de Carvalho, LA Basso, MA Mendes, MS Palma, DS Santos
Biochemical and Biophysical Research Communications 2003 vol. 308 pp. 545-552
Crystal structure of human purine nucleoside phosphorylase complexed with acyclovir DM dos Santos, F Canduri, JH Pereira, MVB Dias, Rafael Guimaraes da Silva, MA Mendes, MS Palma, LA Basso, WF de Azevedo, DS Santos
Biochemical and Biophysical Research Communications 2003 vol. 308 pp. 553-559
Docking and small angle X-ray scattering studies of purine nucleoside phosphorylase WF de Azevedo, G dos Santos, DM dos Santos, JR Olivieri, F Canduri, Rafael Guimaraes da Silva, LA Basso, G Renard, IO Fonseca, MA Mendes, MS Palma, DS Santos
Biochemical and Biophysical Research Communications 2003 vol. 309 pp. 923-928
Structural basis for inhibition of human PNP by immucillin-H WF de Azevedo, F Canduri, DM dos Santos, JH Pereira, MVB Dias, Rafael Guimaraes da Silva, MA Mendes, LA Basso, MS Palma, DS Santos
Biochemical and Biophysical Research Communications 2003 vol. 309 pp. 917–922