Biology Online Module Handbook

BL4210 Practical Skills for Molecular Biology and Biochemistry

(BL4210 online module handbook version 17)

cover image
Practical skills are the core of research in biochemistry and molecular biology. This module is designed to prepare students for laboratory research projects in internationally competitive research. The module is designed to foster skills such as experimental design, core practical skills, data analysis and excellent record keeping. Each practical requires some prior theoretical familiarity. Emphasis is placed upon experimental design - notably anticipation of experimental outcomes and the choice of appropriate experimental controls. This planning phase is followed by execution of the experiment and analyses of the data.*

Credits: 15*

Semester: 1

Module Organiser
Dr Michael M Nevels
mmn3@st-andrews.ac.uk
01334 463375
Pre-requisite Modules:
PERMISSION OF BIOLOGY HONOURS ADVISER REQUIRED*
Anti-requisite Modules:
None*
Post-requisite Modules:
*
Additional Module Information:
Please check MMS regularly for additional module information


Biology Teaching Office:
Room 1.17, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST

Email: bioteach@st-andrews.ac.uk      Tel: 01334  463602

BL4210: Timetable

Legend (not all modules have every event type):
lecture
tutorial
workshop
practical
other

Semester 1: Week 1

DATE & TIMEVENUESTAFFEVENT
Monday
18-09-2017
14:00 to 15:00
Biomolecular Sciences Building
Seminar Room 1.13
Dr Michael M Nevels

Lecture L1: Introduction to the Module | qPCR & Mutagenesis Pre-Plan Advice
2017-8_BL4210_L1
Thursday
21-09-2017
14:00 to 17:00
Bute Building
Computer Classroom A21
Prof Malcolm White

Practical P1: Bioinformatics Practical
2017-8_BL4210_P1
Friday
22-09-2017
14:00 to 17:00
Bute Building
Computer Classroom A21
Prof Malcolm White

Practical P2: Bioinformatics Practical
2017-8_BL4210_P2

Semester 1: Week 4

DATE & TIMEVENUESTAFFEVENT
Monday
09-10-2017
14:00 to 15:00
Biomolecular Sciences Building
Seminar Room 1.13
Dr Michael M Nevels

Lecture L2: qPCR & Mutagenesis Pre-Plan Discussion | Practical Advice
2017-8_BL4210_L2
Tuesday
10-10-2017
10:00 to 17:00
Purdie Building
Microbiology Teaching Laboratory 370
Dr Michael M Nevels
Dr Christina Paulus
Practical P3: qPCR & Mutagenesis Practical
2017-8_BL4210_P3
Thursday
12-10-2017
10:00 to 13:00
Purdie Building
Microbiology Teaching Laboratory 370
Dr Michael M Nevels
Dr Christina Paulus
Practical P4: qPCR & Mutagenesis Practical
2017-8_BL4210_P4
Friday
13-10-2017
14:00 to 17:00
Medical and Biological Sciences Building
Seminar Room 2
Dr Michael M Nevels
Dr Christina Paulus
Practical P5: qPCR & Mutagenesis Class Data Collation | Data Analysis Advice
2017-8_BL4210_P5

Semester 1: Week 5

DATE & TIMEVENUESTAFFEVENT
Friday
20-10-2017
14:00 to 15:00
Biomedical Sciences Building
Seminar Room 1.13
Dr Michael M Nevels

Lecture L3: Cell Transfection Pre-Plan Advice
2017-8_BL4210_L3

Semester 1: Week 9

DATE & TIMEVENUESTAFFEVENT
Monday
13-11-2017
14:00 to 15:00
Biomolecular Sciences Building
Seminar Room 1.13
Dr Michael M Nevels

Lecture L4: Cell Transfection Pre-Plan Discussion | Practical Advice
2017-8_BL4210_L4
Tuesday
14-11-2017
14:00 to 17:00
Purdie Building
Microbiology Teaching Laboratory 370
Dr Michael M Nevels
Dr Christina Paulus
Practical P6: Cell Transfection Practical
2017-8_BL4210_P6
Thursday
16-11-2017
10:00 to 17:00
Purdie Building
Microbiology Teaching Laboratory 370
Dr Michael M Nevels
Dr Christina Paulus
Practical P7: Cell Transfection Practical
2017-8_BL4210_P7
Friday
17-11-2017
14:00 to 17:00
Medical and Biological Sciences Building
Seminar Room 2
Dr Michael M Nevels
Dr Christina Paulus
Practical P8: Cell Transfection Class Data Collation
2017-8_BL4210_P8
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL4210: Reading List

Click for BL4210 reading list

BL4210: Assessment

1-hour Written Examination = 30%, Coursework = 70%*

Assessment:
qPCR & Mutagenesis Pre-plan
Due by:
29/09/2017 13:00
Feedback due by:
09/10/2017 13:00
Type:
Single Upload
Weight:
25%
Assessment:
qPCR & Mutagenesis Report
Due by:
20/10/2017 13:00
Feedback due by:
30/10/2017 13:00
Type:
Single Upload
Weight:
25%
Assessment:
Cell Transfection Pre-plan
Due by:
03/11/2017 13:00
Feedback due by:
13/11/2017 13:00
Type:
Single Upload
Weight:
25%
Assessment:
Cell Transfection Report
Due by:
27/11/2017 13:00
Feedback due by:
07/12/2017 13:00
Type:
Single Upload
Weight:
25%

(MMS assessment data cached: 20 September 2017 22:20:10.)


The following related information applies to all Biology modules:

School of Biology Marking Criteria: See School of Biology Undergraduate Handbook
Late submission of continuous assessment work:

All late submissions of coursework that do not require electronic submission should be made via the late submissions box in the Biomolecular Science Building (beside the Teaching Office)
Exam details: See School of Biology Undergraduate Handbook
Exam timetable: see http://www.st-andrews.ac.uk/students/academic/examinations/examtimetablescurrent/
Expected attendance: See School of Biology Undergraduate Handbook for detailed attendance requirements.
Good Academic Practice & Avoiding Academic Misconduct: See School of Biology Undergraduate Handbook
University Student Handbook: University Student Handbook
School and University regulations in the School and University Undergraduate Handbook relating to absence reporting, penalties and rules for late submission of work, extensions for coursework, return of coursework, S-coding, good academic practice and Academic Alerts.: School of Biology Undergraduate Handbook University Student Handbook
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

Who to ask

(Information in this section applies to all Biology Modules)

Questions about different aspects of the module should be directed to different people:

Questions about Contact
General teaching matters Biology Teaching Office ( bioteach@st-andrews.ac.uk )
Rescheduled or cancelled events Check your University email
Lecture or practical content The lecturer who presented the material
Completing assessed practical assignments The lecturer who set the assignment
Completing assessments Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Marking on continuous assessment The Demonstrator or Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Marking on exams Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Rearranging practical days Grant Brown
Absence and/or extensions Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk) and the Biology Teaching Office ( bioteach@st-andrews.ac.uk )

Difficulties with academic progress which impact more than one module:

Year Coordinator

See School of Biology Undergraduate student handbook for list: http://biology.st-andrews.ac.uk/documents/UndergraduateHandbook.pdf

Overall performance, progress or future directions:

Advisor of Studies

Disability:

Disability Coordinator (Dr Jacqueline Nairn jn37@st-andrews.ac.uk)

Biology Teaching Office:

Room 1.17, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST

Email: bioteach@st-andrews.ac.uk

Tel: 01334 463602

BL4210: Contributing Staff

Dr Michael M Nevels

Dr Michael M Nevels
(Module Organiser)

Reader in Virologymmn3@st-andrews.ac.uk
Dr Michael M NevelsDr Michael M Nevels
(Module Organiser)
Reader in Virologymmn3@st-andrews.ac.uk
Dr Christina PaulusResearch Fellowcp212@st-andrews.ac.uk
Prof Malcolm WhiteProf Malcolm WhiteProfessormfw2@st-andrews.ac.uk
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL4210: Learning Outcomes

Students completing module BL4210 successfully should be able to:

  • Probe databases for relevant information and use bioinformatics tools in biochemistry and molecular biology research projects
  • Design experimental protocols, incorporate appropriate negative/positive experimental controls and anticipate likely experimental outcomes
  • Carry out key modern biochemistry and molecular biology experiments and create clear and complete research records
  • Analyse and interpret experimental data, compare results and draw valid conclusions
  • Write clear and logical scientific prose
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL4210: Acquired Skills

Practical Skills

  • Biomolecule isolation/characterisation
  • Buffers
  • Database interogation
  • DNA isolation
  • Enzyme assay
  • Handling mammalian cells
  • Handling microbes
  • Image analysis
  • Image processing
  • Pipetting
  • Polymerase Chain Reaction (PCR)
  • Protein quantitation
  • Transformation

Transferable Skills

  • "Full" practical write-up (Intro, Methods, Results, Discussion)
  • Critically evaluating sources/information
  • Finding information on the web
  • Referencing
  • Searching databases
  • Lab safety awareness
  • Reflective analysis
  • Calculations/equations
  • Concentrations
  • Data analysis
  • Data analysis (depending on project)
  • Data presentation
  • Descriptive statistics
  • Dilutions
  • Distinguish different types of data
  • Draw a line of best fit
  • Linear regression
  • Produce graphs/figures
  • Produce tables
  • SI units
  • Use Excel
  • Volumes
  • Lab or field notebook
  • Designing experiments
  • Generate class dataset
  • Working in pairs/small groups
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

Policies

(Information in this section applies to all Biology Modules)