School of Biology current student

Information for current students at the School of Biology

Biology Online Module Handbook

BL4210 Practical Skills for Molecular Biology and Biochemistry

(BL4210 online module handbook version 103)

cover image
Practical skills are the core of research in biochemistry and molecular biology. This module is designed to prepare students for laboratory research projects in internationally competitive research. The module is designed to foster skills such as experimental design, core practical skills, data analysis and excellent record keeping. Each practical requires some prior theoretical familiarity. Emphasis is placed upon experimental design - notably anticipation of experimental outcomes and the choice of appropriate experimental controls. This planning phase is followed by execution of the experiment and analyses of the data.*

Credits: 15*

Semester: 1

Module Organiser
Dr Michael M Nevels
mmn3@st-andrews.ac.uk
01334 463375
Pre-requisite Modules:
Permission of Biology Honours Adviser required*
Anti-requisite Modules:
*
Post-requisite Modules:
*
Additional Module Information:


Biology Teaching Office:
Level 2, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST

Email: bioteach@st-andrews.ac.uk      Phone: +44 (0)1334 46 3602 or 3566

BL4210: Timetable

Legend (not all modules have every event type):
lecture
tutorial
workshop
practical
other

Semester 1: Week 1

DATE & TIMEVENUESTAFFEVENT
Monday
11-09-2023
14:00 to 15:00
Medical and Biological Sciences Building
103 Seminar Room 1
Dr Michael M Nevels
-
Lecture L1: Introduction to Module | Mutagenesis & qPCR Pre-plan Advice
2023-4_BL4210_L1
Thursday
14-09-2023
14:00 to 17:00
Biomolecular Sciences Building
205a/b Teaching Lab
Dr Simon Young
-
Practical P1: Bioinformatics Practical I
2023-4_BL4210_P1
Friday
15-09-2023
14:00 to 17:00
Biomolecular Sciences Building
205a/b Teaching Lab
Dr Simon Young
-
Practical P2: Bioinformatics Practical II
2023-4_BL4210_P2

Semester 1: Week 4

DATE & TIMEVENUESTAFFEVENT
Monday
02-10-2023
14:00 to 15:00
Biomolecular Sciences Building
RM001 Seminar Room
Dr Michael M Nevels
-
Lecture L2: Mutagenesis & qPCR Pre-plan Discussion | Practical Advice
2023-4_BL4210_L2
Tuesday
03-10-2023
10:00 to 17:00
Biomolecular Sciences Building
205b Teaching Lab
Dr Michael M Nevels
-
Practical P3: Mutagenesis & qPCR Practical
2023-4_BL4210_P3
Thursday
05-10-2023
10:00 to 13:00
Biomolecular Sciences Building
205a/b Teaching Lab
Dr Michael M Nevels
-
Practical P4: Mutagenesis & qPCR Practical
2023-4_BL4210_P4
Friday
06-10-2023
14:00 to 16:00
Medical and Biological Sciences Building
103 Seminar Room 1
Jason Corbett
Miss Inês Ferreira
Tutorial T1: Mutagenesis & qPCR Class Data Collation | Data Analysis Advice
2023-4_BL4210_T1

Semester 1: Week 5

DATE & TIMEVENUESTAFFEVENT
Friday
13-10-2023
14:00 to 15:00
Biomolecular Sciences Building
RM001 Seminar Room
Dr Michael M Nevels
-
Lecture L3: Cell Transfection Pre-plan Advice
2023-4_BL4210_L3

Semester 1: Week 9

DATE & TIMEVENUESTAFFEVENT
Monday
06-11-2023
14:00 to 15:00
Biomolecular Sciences Building
RM001 Seminar Room
Dr Michael M Nevels
-
Lecture L4: Cell Transfection Pre-plan Discussion | Practical Advice
2023-4_BL4210_L4
Tuesday
07-11-2023
10:00 to 13:00
Biomolecular Sciences Building
205a/b Teaching Lab
Dr Michael M Nevels
-
Practical P5: Cell Transfection Practical
2023-4_BL4210_P5
Thursday
09-11-2023
10:00 to 17:00
Biomolecular Sciences Building
205a/b Teaching Lab
Dr Michael M Nevels
-
Practical P6: Cell Transfection Practical
2023-4_BL4210_P6
Friday
10-11-2023
14:00 to 16:00
Biomolecular Sciences Building
RM001 Seminar Room
Jason Corbett
Miss Inês Ferreira
Tutorial T2: Cell Transfection Class Data Collation | Data Analysis Advice
2023-4_BL4210_T2
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL4210: Assessment

Coursework = 100%*

The following related information applies to all Biology modules:

School of Biology Marking Criteria: See JH booklet info (st-andrews.ac.uk) 
Late submission of continuous assessment work:

All late submissions of coursework that do not require electronic submission should be made via the Biology Teaching Office, Level 2, BMS Building, North Haugh.
Exam details: See School of Biology UG Handbook JH booklet info (st-andrews.ac.uk)  : All Biology exams will be conducted online for 2022-23.
Exam timetable: See Timetables - Exams - University of St Andrews (st-andrews.ac.uk) 
Expected attendance: See JH booklet info (st-andrews.ac.uk)  for detailed attendance requirements.
Good Academic Practice & Avoiding Academic Misconduct: See JH booklet info (st-andrews.ac.uk)
University Student Handbook: University Student Handbook
School and University regulations in the School and University Undergraduate Handbook relating to absence reporting, penalties and rules for late submission of work, extensions for coursework, return of coursework, S-coding, good academic practice and Academic Alerts.: JH booklet info (st-andrews.ac.uk)
University Student Handbook
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

Who to ask

(Information in this section applies to all Biology Modules)

Before contacting staff, students should check the content of the Biology Undergraduate Handbook, the module handbook and specific task instructions.

Questions about Contact
General teaching matters Biology Teaching Office ( bioteach@st-andrews.ac.uk )
Rescheduled or cancelled events Check your University email
Lecture or practical content The lecturer who presented the material
Completing assessed practical assignments The lecturer who set the assignment
Completing assessments Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Marking on continuous assessment The Demonstrator or Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Marking on exams Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Rearranging practical days
Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)
Absence and/or extensions Module Organiser (Dr Michael M Nevels mmn3@st-andrews.ac.uk)

and the Biology Teaching Office ( bioteach@st-andrews.ac.uk )

Difficulties with academic progress which impact more than one module:

Year Coordinator

See School of Biology UG Handbook for list:
JH booklet info (st-andrews.ac.uk)

Overall performance, progress or future directions:

Advisor of Studies

Disability:

Disability Coordinator ( biodisabilities@st-andrews.ac.uk )

For advice and support on any issue e.g. academic, financial, international, personal or health matters, or if you are unsure of who to go to for help:

Advice & Support Centre

Address: 79 North Street, St Andrews

Email: theasc@st-andrews.ac.uk

Web: https://www.standrews.ac.uk/ask-a-question/

Tel: 01334 462020

University assistance with urgent matters out of office hours:

Tel: 01334 476161

Web: https://www.st-andrews.ac.uk/students/advice/counselling/incrisis/

Biology Teaching Office:

We are happy to hear from you about teaching matters. The School of Biology Teaching Office is open Monday to Friday 09.00 - 13.00 and 14.00 - 17.00. School of Biology staff will respond to your emails during these hours. Our team will provide a response to you within three working days.

Biology Teaching Office (Level 2), University of St Andrews, Biomolecular Sciences Building, North Haugh, St Andrews, Fife KY16 9ST

Email: bioteach@st-andrews.ac.uk

Tel: 01334 46 3602 or 3566

BL4210: Contributing Staff

Dr Michael M Nevels
(Module Organiser)

Reader in Virologymmn3@st-andrews.ac.uk
Jason CorbettPostgraduate Studentjwc7@st-andrews.ac.uk
Miss Inês FerreiraPostgraduate Studentiaf1@st-andrews.ac.uk
Dr Michael M Nevels
(Module Organiser)
Reader in Virologymmn3@st-andrews.ac.uk
Dr Simon YoungAssociate Lecturer (Education focused)say2@st-andrews.ac.uk
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL4210: Learning Outcomes

Students completing module BL4210 successfully should be able to:

  • Probe databases for relevant information and use bioinformatics tools in biochemistry and molecular biology research projects
  • Design experimental protocols, incorporate appropriate negative/positive experimental controls and anticipate likely experimental outcomes
  • Carry out key modern biochemistry and molecular biology experiments and create clear and complete research records
  • Analyse and interpret experimental data, compare results and draw valid conclusions
  • Write clear and logical scientific prose
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

BL4210: Acquired Skills

Practical Skills

  • Biomolecule isolation/characterisation
  • Buffers
  • Database interogation
  • DNA isolation
  • Enzyme assay
  • Handling mammalian cells
  • Handling microbes
  • Image analysis
  • Image processing
  • Pipetting
  • Polymerase Chain Reaction (PCR)
  • Protein quantitation
  • Transformation

Transferable Skills

  • "Full" practical write-up (Intro, Methods, Results, Discussion)
  • Critically evaluating sources/information
  • Finding information on the web
  • Referencing
  • Searching databases
  • Lab safety awareness
  • Reflective analysis
  • Calculations/equations
  • Concentrations
  • Data analysis
  • Data analysis (depending on project)
  • Data presentation
  • Descriptive statistics
  • Dilutions
  • Distinguish different types of data
  • Draw a line of best fit
  • Linear regression
  • Produce graphs/figures
  • Produce tables
  • SI units
  • Use Excel
  • Volumes
  • Lab or field notebook
  • Designing experiments
  • Generate class dataset
  • Working in pairs/small groups
Data Sources: information in this online module handbook is gathered from several sources.
Small footnote marks beside content, or by section titles indicate the source.
* University of St Andrews Module Catalogue
School of Biology Symbiosis Database (module organisers only)
University of St Andrews MMS (St Andrews users only)

Policies

(Information in this section applies to all Biology Modules)

  • The procedures and regulations followed by the School of Biology are outlined in the University Handbook and in the School of Biology UG handbook  JH booklet info (st-andrews.ac.uk) 
  • All coursework associated with the module must be completed and submitted by its due date.
  • Specific School regulations relating to absence reporting, penalties and rules for late submission of work, extensions for coursework, return of coursework, S-coding, Good Academic Practice and Academic Alert are stated in the School of Biology UG hand book JH booklet info (st-andrews.ac.uk)  and students are required to carefully read these regulations.
  • Students are also referred to the University Handbook, available at: http://www.st-andrews.ac.uk/studenthandbook/